Journal: Stem Cell Reports

Article Title: Differentiation of brain and retinal organoids from confluent cultures of pluripotent stem cells connected by nerve-like axonal projections of optic origin

doi: 10.1016/j.stemcr.2022.04.003

Figure Lengend Snippet: Cortical organoids develop synchronous networks (A) Cortical organoid plated overnight on multielectrode array (MEA). (B and C) Representative spontaneous firing activity of organoid. (D and E) Spike raster plots showing firing patterns of organoids across all electrodes with marked network bursts. (F) TTX treatment abolished firing activity, which returned following washing (mean ± SD, paired two-tailed t test, ∗∗∗∗ p < 0.0001). (G) Heatmap showing proteome comparison of iPSC, organoids, 4 months, and 23-year-old brain. (H) Proteins differentially enriched in organoids cultured in CODM or BrainPhys media (p < 0.05, adjusted by a false discovery rate of 5%). See also Figure S4 .

Article Snippet: At 10 weeks, cortical organoids were cultured in a cerebral organoid differentiation medium as described in (50% neurobasal medium, 50% DMEM/F12, 0.5% N 2 supplement, 0.03% insulin, 1% GlutaMAX, MEM-NEAA, B-mercaptoethanol, 1% B27 supplement) or BrainPhys hPSC Neuron Kit (Stem Cell Technologies, BrainPhys Neuronal medium, NeuroCult SM1 neuronal supplement, 1% N 2 supplement, human recombinant BDNF and GDNF, ascorbic acid and dibutyryl-cAMP). iPSC line UCLOOi017-A-1 was used in most of the main figures.

Techniques: Activity Assay, Two Tailed Test, Comparison, Cell Culture